Antibody is a kind of immune globulin secreted by B lymphocyte, which can specifically bind to the antigen. Antibodies are divided into monoclonal antibodies and polyclonal antibodies, which widely used in biology and medicine, disease prevention, medical diagnosis and scientific research.
The main steps in the preparation of monoclonal antibodies are:
Normal mouse immunization;
Physical, chemical and biological methods to promote cell fusion;
Hybridoma cells screening and culture;
Monoclonal antibody purification.
1. Protein antigen design and expression;
2. Normal immune treatment;
3. Antigen affinity purification;
4. Efficacy testing